| 出品公司: | BD Biosciences |
|---|---|
| 载体名称: | pAcGHLT B |
| 质粒类型: | 昆虫细胞载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 8755 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pAcGHLTB载体基本信息
pAcGHLTB载体图谱质粒图谱和多克隆位点信息
pAcGHLTB载体简介
pAcGHLTB载体序列
LOCUS pAcGHLT B 8755 bp ds-DNA circular SYN 19-APR-2014
DEFINITION Baculovirus transfer vector for expressing proteins with an
N-terminal GST-6xHis-PKA-thrombin cassette. For other reading
frames, use pAcGHLT A or pAcGHLT C.
ACCESSION .
VERSION .
KEYWORDS pAcGHLT B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8755)
AUTHORS BD Biosciences
TITLE Direct Submission
JOURNAL Exported Thursday, February 6, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8755
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
misc_recomb 1269..2096
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 1269..1393
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(1431..2036)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
promoter 2100..2191
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
CDS 2206..2859
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/note="GST"
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 2875..2892
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 2899..2916
/codon_start=1
/product="recognition site for phosphorylation by protein
kinase A"
/note="PKA site"
/translation="RRASVA"
CDS 2926..2943
/codon_start=1
/product="thrombin recognition and cleavage site"
/note="thrombin site"
/translation="LVPRGS"
misc_feature 2966..3027
/note="MCS"
/note="multiple cloning site"
misc_recomb 3047..4505
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(3164..4505)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin 5878..6333
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6615..6719
/gene="bla"
/note="AmpR promoter"
CDS 6720..7580
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7751..8339
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatccgat gggacatcat
2881 catcatcatc acggaaggag aagggccagt gttgcggcgg gaattttggt ccctcgtgga
2941 agcccaggac tcgatggcat atatgctcga ggaattcagg cctccatggg agctcgcggc
3001 cgcctgcagg gtacccccgg gagatctgta ccgactctgc tgaagaggag gaaattctcc
3061 ttgaagtttc cctggtgttc aaagtaaagg agtttgcacc agacgcacct ctgttcactg
3121 gtccggcgta ttaaaacacg atacattgtt attagtacat ttattaagcg ctagattctg
3181 tgcgttgttg atttacagac aattgttgta cgtattttaa taattcatta aatttataat
3241 ctttagggtg gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt
3301 taaatattaa atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt
3361 gtttttccga accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg
3421 ccaaatcttg tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata
3481 aaggttcgac gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag
3541 tgtacaattg actcgacgta aacacgttaa ataaagcttg gacatattta acatcgggcg
3601 tgttagcttt attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt
3661 ccgaagacga ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg
3721 cgatcaaatt tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg
3781 gtttcaatct aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag
3841 gcggtggtaa catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata
3901 aatctaccat cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg
3961 gcggtgatgc agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct
4021 caactattgt actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat
4081 ttttttcgtt tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt
4141 gaggttccgt cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg
4201 gtggaggcgc tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa
4261 tttgttctgg tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct
4321 gcacaacgga aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat
4381 aattggaata caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg
4441 tgccgatatt taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc
4501 tcggatcgat cccgcacgcc gataacaagc cttttcattt ttactacagc attgtagtgg
4561 cgagacactt cgctgtcgtc gacgtacatg tatgctttgt tgtcaaaaac gtcgttggca
4621 agctttaaaa tatttaaaag aacatctctg ttcagcacca ctgtgttgtc gtaaatgttg
4681 tttttgataa tttgcgcttc cgcagtatcg acacgttcaa aaaattgatg cgcatcaatt
4741 ttgttgttcc tattattgaa taaataagat tgtacagatt catatctacg attcgtcatg
4801 gccaccacaa atgctacgct gcaaacgctg gtacaatttt acgaaaactg caaaaacgtc
4861 aaaactcggt ataaaataat caacgggcgc tttggcaaaa tatctatttt atcgcacaag
4921 cccactagca aattgtattt gcagaaaaca atttcggcgc acaattttaa cgctgacgaa
4981 ataaaagttc accagttaat gagcgaccac ccaaatttta taaaaatcta ttttaatcac
5041 ggttccatca acaaccaagt gatcgtgatg gactacattg actgtcccga tttatttgaa
5101 acactacaaa ttaaaggcga gctttcgtac caacttgtta gcaatattat tagacagctg
5161 tgtgaagcgc tcaacgattt gcacaagcac aatttcatac acaacgacat aaaactcgaa
5221 aatgtcttat atttcgaagc acttgatcgc gtgtatgttt gcgattacgg attgtgcaaa
5281 cacgaaaact cacttagcgt gcacgacggc acgttggagt attttagtcc ggaaaaaatt
5341 cgacacacaa ctatgcacgt ttcgtttgac tggtacgcgg cgtgttaaca tacaagttgc
5401 taaccggcgg ccgacaccca tttgaaaaaa gcgaagacga aatgttggac ttgaatagca
5461 tgaagcgtcg tcagcaatac aatgacattg gcgttttaaa acacgttcgt aacgttaacg
5521 ctcgtgactt tgtgtactgc ctaacaagat acaacataga ttgtagactc acaaattaca
5581 aacaaattat aaaacatgag tttttgtcgt aaaaatgcca cttgttttac gagtagaatt
5641 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
5701 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
5761 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
5821 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
5881 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
5941 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
6001 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
6061 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
6121 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
6181 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
6241 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
6301 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
6361 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
6421 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
6481 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
6541 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
6601 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
6661 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
6721 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
6781 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
6841 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
6901 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
6961 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
7021 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
7081 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
7141 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
7201 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
7261 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
7321 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
7381 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
7441 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
7501 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
7561 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
7621 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
7681 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
7741 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
7801 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
7861 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
7921 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
7981 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
8041 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
8101 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
8161 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
8221 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
8281 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
8341 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
8401 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
8461 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
8521 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
8581 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
8641 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
8701 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaatt
//
